کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1952441 1057210 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
DNA condensation in bacteria: Interplay between macromolecular crowding and nucleoid proteins
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
DNA condensation in bacteria: Interplay between macromolecular crowding and nucleoid proteins
چکیده انگلیسی

The volume of a typical Eschericia coli nucleoid is roughly 104 times smaller than the volume of a freely coiling linear DNA molecule with the same length as the E. coli genome. We review the main forces that have been suggested to contribute to this compaction factor: macromolecular crowding (that “pushes” the DNA together), DNA charge neutralization by various polycationic species (that “glues” the DNA together), and finally, DNA deformations due to DNA supercoiling and nucleoid proteins. The direct contributions of DNA supercoiling and nucleoid proteins to the total compaction factor are probably small. Instead, we argue that the formation of the bacterial nucleoid can be described as a consequence of the influence of macromolecular crowding on thick, supercoiled protein-DNA fibers, that have been partly charge neutralized by small multivalent cations.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimie - Volume 92, Issue 12, December 2010, Pages 1715–1721
نویسندگان
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