کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1952503 | 1057212 | 2011 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Glutathionyl-biliverdin IXα, a new heme catabolite in a marine annelid: Sex and cell specific accumulation
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کلمات کلیدی
CIDESI-MSTFAVitellogeninDiI - DIIROS - ROSElectrospray Ionization Mass Spectrometry - اسپکترومتر جرم یونیزاسیون ElectrosprayTrifluoroacetic acid - اسید TrifluoroaceticBiliverdin - بیلیاردینcollision induced dissociation - تقارن ناشی از برخوردAbsorption - جذبPacked cell volume - حجم سلول بسته بندی شدهTandem mass spectrometry - طیف سنجی جرمی پشت سر هم یا متوالیPCV یا Pneumococcal conjugate vaccine - واکسن کونژوگه پنوموکوکGlutathione - گلوتاتیونReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We have isolated and characterized the green pigment accumulating in coelomic cells (eleocytes) of the common clam worm Nereis virens by means of RP-HPLC and ESI-tandem mass spectrometry. This pigment is a novel biliverdin-glutathione conjugate in which the glutathione is linked to the biliverdin-backbone via a thioether bond. The yolk precursor vitellogenin, a female-specific high-density lipoprotein (ÏÂ =Â 1179Â kg/m3) with a native molecular mass of â¼500Â kDa and a subunit mass of â¼150Â kDa, is capable of transporting this pigment as well as heme. The sex-independent large discoidal lipoprotein present in the coelomic fluid, which is sequestered by the eleocytes, could also be shown to transport heme. This renders both lipoproteins as heme-lipoproteins. As the vitellogenin is secreted by the eleocytes, this suggests the eleocytes as a metabolic hub linking the uptake of the potent pro-oxidant heme thought to arise from aged hemoglobin via the large discoidal lipoprotein and its conversion to a bile pigment-conjugate. The conjugate is exported from the eleocytes to the oocytes via vitellogenin leading to the accumulation of green yolk protein. In contrast, no such export route exists in male eleocytes resulting in an accumulation of the biliverdin-conjugate in these cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimie - Volume 93, Issue 2, February 2011, Pages 207-216
Journal: Biochimie - Volume 93, Issue 2, February 2011, Pages 207-216
نویسندگان
Sven Schenk, Ulrich Hoeger,