Molecular cloning and characterization of neutral ceramidase homologue from the red flour beetle, Tribolium castaneum

► Tribolium castaneum ceramidase (Tncer) is mainly localized to the plasma membrane. ► Its mRNA levels are much higher in ovary. ► Tncer has a much broader pH optimum than any other known neutral ceramidases. ► Tncer's substrate specificity is broader than mammalian ceramidases.