کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1953228 1057257 2008 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Degradation of plastid unspliced transcripts and lariat group II introns
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Degradation of plastid unspliced transcripts and lariat group II introns
چکیده انگلیسی

Group II introns are widespread among plant organellar genomes. Despite several investigations, knowledge about the structural features of group II introns in relation to the possible mechanism of splicing after releasing is still scarce. In the present paper, we have analyzed degradation products of the ndhA, atpF, and petB transcripts, demonstrating the existence of both incomplete introns and unspliced pre-mRNAs, which presumably correspond with their respective intermediate degradation products. Nucleotide sequencing of both 5′ and 3′ ends of such RNA species showed, for the first time in plant transcripts, that cleavages affect specific intron domains and occur within an unpaired bubble flanked by two-stem structures typical for prokaryotic RNAse III processing sites. Degradation of both unspliced pre-mRNAs and lariat introns is also proposed as an additional mechanism that controls the level of mature translatable mRNAs of chloroplast genes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimie - Volume 90, Issue 3, March 2008, Pages 474–483
نویسندگان
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