کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1953557 1057289 2006 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel butyrylcholinesterase from serum of Leporinus macrocephalus, a Neotropical fish
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
A novel butyrylcholinesterase from serum of Leporinus macrocephalus, a Neotropical fish
چکیده انگلیسی

We show here that serum of piaussu, a Neotropical characin fish, has the highest butyrylcholinesterase activity so far described for humans and fish. To clarify whether this cholinesterase could protect piaussu against anticholinesterase pesticides by scavenging organophosphates, we purified it 1700-fold, with a yield of 80%. Augmenting concentrations (from 0.01 to 20 mM) of butyrylthiocholine activated it. The pure enzyme was highly inhibited by chlorpyriphos-oxon (ki = 10,434 × 106 M−1 min−1) and by the specific butyrylcholinesterase inhibitor, isoOMPA (ki = 45.7 × 106 M−1 min−1). Electrophoresis of total serum and 2-D electrophoresis of the purified cholinesterase showed that some enzyme molecules could circulate in piaussu serum as heterogeneously glycosylated dimers. The enzyme's N-terminal sequence was similar to sequences found for butyrylcholinesterase from sera of other vertebrates. Altogether, our data present a novel butyrylcholinesterase with the potential of protecting a fish from poisoning by organophosphates.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimie - Volume 88, Issue 1, January 2006, Pages 59–68
نویسندگان
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