کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1955595 1057828 2007 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Formation of Ceramide/Sphingomyelin Gel Domains in the Presence of an Unsaturated Phospholipid: A Quantitative Multiprobe Approach
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Formation of Ceramide/Sphingomyelin Gel Domains in the Presence of an Unsaturated Phospholipid: A Quantitative Multiprobe Approach
چکیده انگلیسی

To better understand how ceramide modulates the biophysical properties of the membrane, the interactions between palmitoyl-ceramide (PCer) and palmitoyl-sphingomyelin (PSM) were studied in the presence of the fluid phospholipid palmitoyl-oleoyl-phosphatidylcholine (POPC) in membrane model systems. The use of two fluorescent membrane probes distinctly sensitive to lipid phases allowed a thorough biophysical characterization of the ternary system. In these mixtures, PCer recruits POPC and PSM in the fluid phase to form extremely ordered and compact gel domains. Gel domain formation by low PCer mol fraction (up to 12 mol %) is enhanced by physiological PSM levels (∼20–30 mol % total lipid). For higher PSM content, a three-phase situation, consisting of fluid (POPC-rich)/gel (PSM-rich)/gel (PCer-rich) coexistence, is clearly shown. To determine the fraction of each phase a quantitative method was developed. This allowed establishing the complete ternary phase diagram, which helps to predict PCer-rich gel domain formation and explains its enhancement through PSM/PCer interactions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 93, Issue 5, 1 September 2007, Pages 1639–1650
نویسندگان
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