کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1956669 1057865 2008 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fluorescence Correlation Spectroscopy of Finite-Sized Particles
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Fluorescence Correlation Spectroscopy of Finite-Sized Particles
چکیده انگلیسی

A theory is presented to study fluorescence correlation spectroscopy for particles with size comparable to the beam waist of the observation volume. Analytical correlation curves are derived for some experimentally interesting particle geometries. It is found that the finiteness of the particle generally decreases the value of the correlation amplitude and increases the correlation time compared to a point particle model. Furthermore, not only the size but also the distribution of fluorophores affects the shape of the correlation function. This is experimentally demonstrated with surface and internally labeled fluorescent spheres. In addition, experiments are performed on fluorescent spheres of different radii to validate the model by comparing the results to theoretical predictions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 94, Issue 7, April 2008, Pages 2800–2808
نویسندگان
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