کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1957291 1057878 2005 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Direct Visualization of Asymmetric Behavior in Supported Lipid Bilayers at the Gel-Fluid Phase Transition
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Direct Visualization of Asymmetric Behavior in Supported Lipid Bilayers at the Gel-Fluid Phase Transition
چکیده انگلیسی

We utilize in situ, temperature-dependent atomic force microscopy to examine the gel-fluid phase transition behavior in supported phospholipid bilayers constructed from 1,2-dimyristoyl-sn-glycero-3-phosphocholine, 1,2-dipentadecanoyl-sn-glycero-3-phosphocholine, and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine. The primary gel-fluid phase transition at Tm occurs through development of anisotropic cracks in the gel phase, which develop into the fluid phase. At ∼5°C above Tm, atomic force microscopy studies reveal the presence of a secondary phase transition in all three bilayers studied. The secondary phase transition occurs as a consequence of decoupling between the two leaflets of the bilayer due to enhanced stabilization of the lower leaflet with either the support or the water entrained between the support and the bilayer. Addition of the transmembrane protein gramicidin A or construction of a highly defected gel phase results in elimination of this decoupling and removal of the secondary phase transition.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 88, Issue 3, March 2005, Pages 2154–2164
نویسندگان
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