کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1958974 1057923 2006 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Weak Force Stalls Protrusion at the Leading Edge of the Lamellipodium
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Weak Force Stalls Protrusion at the Leading Edge of the Lamellipodium
چکیده انگلیسی

Protrusion, the first step of cell migration, is driven by actin polymerization coupled to adhesion at the cell’s leading edge. Polymerization and adhesive forces have been estimated, but the net protrusion force has not been measured accurately. We arrest the leading edge of a moving fish keratocyte with a hydrodynamic load generated by a fluid flow from a micropipette. The flow arrests protrusion locally as the cell approaches the pipette, causing an arc-shaped indentation and upward folding of the leading edge. The effect of the flow is reversible upon pipette removal and dependent on the flow direction, suggesting that it is a direct effect of the external force rather than a regulated cellular response. Modeling of the fluid flow gives a surprisingly low value for the arresting force of just a few piconewtons per micrometer. Enhanced phase contrast, fluorescence, and interference reflection microscopy suggest that the flow does not abolish actin polymerization and does not disrupt the adhesions formed before the arrest but rather interferes with weak nascent adhesions at the very front of the cell. We conclude that a weak external force is sufficient to reorient the growing actin network at the leading edge and to stall the protrusion.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 90, Issue 5, 1 March 2006, Pages 1810–1820
نویسندگان
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