کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1959157 1057928 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of Average Phospholipid Curvature on Supported Bilayer Formation on Glass by Vesicle Fusion
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Effect of Average Phospholipid Curvature on Supported Bilayer Formation on Glass by Vesicle Fusion
چکیده انگلیسی

The adsorption of large unilamellar vesicles composed of various combinations of phosphatidylcholine, phosphatidylethanolamine (PE), monomethyl PE, and dimethyl PE (PE-Me2) onto a glass surface was studied using fluorescence microscopy. The average lipid geometry within the vesicles, described mathematically by the average intrinsic curvature, C0,ave, was methodically altered by changing the lipid ratios to determine the effect of intrinsic curvature on the ability of vesicles to rupture and form a supported lipid bilayer. We show that the ability of vesicles to create fluid planar bilayers is dependent on C0,ave and independent of the identity of the component lipids. When the C0,ave was ∼−0.1 nm−1, the vesicles readily formed supported lipid bilayers with almost full mobility. In contrast, when the C0,ave ranged from ∼−0.2 to ∼−0.3 nm−1, the adsorbed vesicles remained intact upon the surface. The results indicate that the average shape of lipid molecules within a vesicle (C0,ave) is essential for determining kinetically viable reactions that are responsible for global geometric changes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 90, Issue 4, 15 February 2006, Pages 1241–1248
نویسندگان
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