کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1960070 1057950 2005 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Elementary Steps of the Cross-Bridge Cycle in Fast-Twitch Fiber Types from Rabbit Skeletal Muscles
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Elementary Steps of the Cross-Bridge Cycle in Fast-Twitch Fiber Types from Rabbit Skeletal Muscles
چکیده انگلیسی

To understand the molecular mechanism underlying the diversity of mammalian skeletal muscle fibers, the elementary steps of the cross-bridge cycle were investigated in three fast-twitch fiber types from rabbit limb muscles. Skinned fibers were maximally Ca2+-activated at 20°C and the effects of MgATP, phosphate (P, Pi), and MgADP were studied on three exponential processes by sinusoidal analysis. The fiber types (IIA, IID, and IIB) were determined by analyzing the myosin heavy-chain isoforms after mechanical experiments using high-resolution SDS-PAGE. The results were consistent with the following cross-bridge scheme: where A is actin, M is myosin, D is MgADP, and S is MgATP. All states except for those in brackets are strongly bound states. All rate constants of elementary steps (k2, 198–526 s−1; k−2, 51–328 s−1; k4, 13.6–143 s−1; k−4, 13.6–81 s−1) were progressively larger in the order of type IIA, type IID, and type IIB fibers. The rate constants of a transition from a weakly bound state to a strongly bound state (k−2, k4) varied more among fiber types than their reversals (k2, k−4). The equilibrium constants K1 (MgATP affinity) and K2 (=k2/k−2, ATP isomerization) were progressively less in the order IIA, IID, and IIB. K4 (=k4/k−4, force generation) and K5 (Pi affinity) were larger in IIB than IIA and IID fibers. K1 showed the largest variation indicating that the myosin head binds MgATP more tightly in the order IIA (8.7 mM−1), IID (4.9 mM−1), and IIB (0.84 mM−1). Similarly, the MgADP affinity (K0) was larger in type IID fibers than in type IIB fibers.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 89, Issue 5, November 2005, Pages 3248–3260
نویسندگان
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