کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1960193 1057952 2006 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Conical Electron Tomography of a Chemical Synapse: Vesicles Docked to the Active Zone are Hemi-Fused
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Conical Electron Tomography of a Chemical Synapse: Vesicles Docked to the Active Zone are Hemi-Fused
چکیده انگلیسی

We have used thin sectioning and conical electron tomography to determine the three-dimensional structure of synaptic vesicles that were associated (docked) at release sites of the presynaptic membrane, called active-zones. Vesicles docked at the active zone occupied a strategic location: they formed regions of contact with the plasma membrane on one side and with that of one or more vesicles located deeper within the presynaptic terminal on the other side. The region of contact with the active zone measured ∼15 nm in diameter (∼2% of the vesicle’s surface) and contained a smaller ∼6 nm region where the proximal leaflets merged (hemi-fused). Hemi-fusion was only observed on the side of vesicles in contact with the active zone; at the side of contact between neighboring vesicles, the membranes were not hemi-fused. Approximately three-fourths of the docked vesicles contained hemi-fused regions. Vesicles fully fused to the active zone (exhibiting pores that appeared as interruptions of a single membrane) were less frequently observed (∼1 of 10 hemi-fused vesicles). In conclusion, our observations in cortical synapses strengthen the hypothesis that hemi-fusion is a stable intermediary that precedes full fusion and release.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 91, Issue 8, 15 October 2006, Pages 2910–2918
نویسندگان
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