کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1964644 1058563 2007 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Murine macrophage P2X7 receptors support rapid prothrombotic responses
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Murine macrophage P2X7 receptors support rapid prothrombotic responses
چکیده انگلیسی

Non-apoptotic externalization of phosphatidylserine (PS) can act as a reactive surface for the efficient assembly of the prothrombinase complex leading to thrombin generation and coagulation. Here we show that extracellular ATP, acting at the macrophage P2X7 receptor, drives the rapid Ca2+-dependent formation and release of PS-rich microvesicles that enhance the assembly of the prothrombinase complex and subsequent formation of thrombin. Incubation with P2X7 receptor antagonists (KN-62 and Brilliant Blue G) attenuates ATP induced prothrombotic responses. Consistent with the hypothesis that exposed PS enhances prothrombinase activity; pre-incubation with annexin V blocks the increase in thrombin formation. The rapid translocation of PS and formation of pro-thrombotic microvesicles occurs in the absence of cell lysis. These data demonstrate that the pro-inflammatory P2X7 receptor can also support and propagate rapid increases in thrombin formation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Signalling - Volume 19, Issue 4, April 2007, Pages 855–866
نویسندگان
, ,