کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1964808 | 1058598 | 2010 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: AMP-activated protein kinase is required for induction of apoptosis and epithelial-to-mesenchymal transition AMP-activated protein kinase is required for induction of apoptosis and epithelial-to-mesenchymal transition](/preview/png/1964808.png)
AMP-activated protein kinase (AMPK) is a serine/threonine protein kinase which has been implicated in the regulation of cellular energy homeostasis. Relatively very little is known about its role in other cellular processes. We observed that AMPK-α can be activated by transforming growth factor-β1 (TGF-β1) in mouse hepatocytes. Inhibition of AMPK by Compound C, a selective AMPK-α inhibitor, inhibited TGF-β1-induced apoptosis and EMT in hepatocytes. In addition, overexpression of a dominant-negative form of AMPK-α subunit also suppressed TGF-β1-induced EMT and apoptosis in AML12 cells. Furthermore, inhibition of AMPK suppressed TGF-β1-induced Smad3 transcriptional activity. This study indicates that AMPK is able to modulate Smad3 transcriptional activity, which plays an important role in TGF-β1-induced apoptosis and EMT.
Journal: Cellular Signalling - Volume 22, Issue 11, November 2010, Pages 1790–1797