کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1965229 1538648 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Testosterone, free testosterone, and free androgen index in women: Reference intervals, biological variation, and diagnostic value in polycystic ovary syndrome
ترجمه فارسی عنوان
تستوسترون، تستوسترون آزاد و شاخص آندروژن رایگان در زنان: فاصله مرزی، تغییرات بیولوژیکی و ارزش تشخیصی در سندرم تخمدان پلی کیستیک
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


• We calculated female reference intervals for testosterone, free testosterone, and androgen index.
• Female biologic variation for testosterone, free testosterone, and androgen index was determined.
• Free androgen index and free testosterone were the strongest predictors of PCOS.

ObjectiveThe objective of our study was to determine reference intervals and biologic variation for testosterone (T), free testosterone (fT), and free androgen index (FAI) in women with accurate methods and to test the discriminative value of these parameters in a polycystic ovary syndrome (PCOS)-population.MethodsSerum was obtained daily during a normal menstrual cycle from 25 healthy women (677 data-points). A single serum sample was obtained from 44 PCOS-patients. T was measured by LC–MS/MS and by Architect® 2nd generation T Immunoassay. Sex hormone-binding globulin was measured to calculate fT and FAI.ResultsReference intervals which were established in healthy women with an ovulatory menstrual cycle were T = 0.3–1.6 nmol/L and 0.5–2.0 nmol/L, fT = 5.2–26 pmol/L and 7.2–33 pmol/L, and FAI = 0.4–2.9 and 0.6–4.4, by LC–MS/MS and immunoassay, respectively. T, fT and FAI were higher in PCOS patients than in controls (p < 0.0001). The areas under the curve of receiver operator characteristic (ROC) plots were not different for T, fT, or FAI when T was measured by LC–MS/MS versus immunoassay based on prediction of PCOS. FAI and fT were the strongest predictors of PCOS.ConclusionsWhen based upon the appropriate reference intervals and ROC analysis, LC–MS/MS and second generation immunoassay have equivalent clinical utility for the diagnosis of PCOS.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinica Chimica Acta - Volume 450, 23 October 2015, Pages 227–232
نویسندگان
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