کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1965703 | 1538680 | 2013 | 5 صفحه PDF | دانلود رایگان |

BackgroundAbnormal gastrointestinal permeability has been linked to irritable bowel syndrome (IBS). The lactulose-to-mannitol ratio is traditionally used to assess small intestine permeability while sucralose and sucrose are used to assess colonic and gastric permeability respectively. We used a single 4-probe test solution to assess permeability throughout the gastrointestinal tract in IBS patients and healthy controls by measuring the recovery of the probes in urine after ingestion using a modified liquid chromatography mass spectrometry protocol.MethodsFasting participants (N = 59) drank a permeability test solution (100 ml: sucralose, sucrose, mannitol, and lactulose). Urine was collected over a 5-h period and kept frozen until analysis. Urinary sugar concentrations were measured using a liquid chromatography/triple quadruple mass spectrometer.ResultsColonic permeability was significantly lower in IBS patients when compared to healthy controls (p = 0.011). Gastric and small intestinal permeability did not significantly differ between the groups.ConclusionsThe study demonstrates the clinical potential of this non-invasive method for assessing alterations in gastrointestinal permeability in patients with IBS.
► Recovery of sugar probes accurately measured using a HLPC–mass spectrometry assay.
► Raffinose was used as an internal standard.
► IBS patients exhibited decreased colonic permeability compared to healthy controls.
Journal: Clinica Chimica Acta - Volume 418, 15 March 2013, Pages 97–101