کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1965733 1538712 2010 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Pleural fluid: Are temperature and storage time critical preanalytical error factors in biochemical analyses?
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Pleural fluid: Are temperature and storage time critical preanalytical error factors in biochemical analyses?
چکیده انگلیسی

BackgroundBiochemical analysis of fluid is the primary laboratory approach in pleural effusion diagnosis. Standardization of the steps between collection and laboratorial analyses are fundamental to maintain the quality of the results. We evaluated the influence of temperature and storage time on sample stability.MethodsPleural fluid from 30 patients was submitted to analyses of proteins, albumin, lactic dehydrogenase (LDH), cholesterol, triglycerides, and glucose. Aliquots were stored at 21°, 4°, and−20 °C, and concentrations were determined after 1, 2, 3, 4, 7, and 14 days. LDH isoenzymes were quantified in 7 random samples.ResultsDue to the instability of isoenzymes 4 and 5, a decrease in LDH was observed in the first 24 h in samples maintained at − 20 °C and after 2 days when maintained at 4 °C. Aside from glucose, all parameters were stable for up to at least day 4 when stored at room temperature or 4 °C.ConclusionsTemperature and storage time are potential preanalytical errors in pleural fluid analyses, mainly if we consider the instability of glucose and LDH. The ideal procedure is to execute all the tests immediately after collection. However, most of the tests can be done in refrigerated samples, excepting LDH analysis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinica Chimica Acta - Volume 411, Issues 17–18, 6 September 2010, Pages 1275–1278
نویسندگان
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