کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1965906 | 1538688 | 2012 | 5 صفحه PDF | دانلود رایگان |
The enzyme-linked immunosorbent spot (ELISPOT) assay for interferon gamma has been available for more than twenty years and has been used for a number of applications, including the monitoring of T cell immunity in solid organ transplant recipients. Studies from single centers indicate that heightened T cell alloreactivity measured with this assay correlates with acute and chronic rejection and with poor long-term allograft function. The assay has been used not only to assess T cell reactivity after transplantation, but also as a tool for assessing immune risk prior to transplantation. Additional work is needed to validate the assay in larger multicenter clinical trials.
► Using donor cells as stimulators, the ELISPOT IFN-γ assay reflects donor-specific alloreactivity.
► High frequencies of IFN-γ producing T cells early after transplant correlate with poor graft function.
► A positive pretransplant donor-specific ELISPOT IFN-γ assay correlates with acute rejection.
► Alloreactivity based on the ELISPOT IFN-γ assay increases with exposure to hemodilaysis.
Journal: Clinica Chimica Acta - Volume 413, Issues 17–18, 8 September 2012, Pages 1359–1363