کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1966034 | 1538709 | 2010 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Rapid detection of JAK2 V617F mutation using high-resolution melting analysis with LightScanner platform Rapid detection of JAK2 V617F mutation using high-resolution melting analysis with LightScanner platform](/preview/png/1966034.png)
BackgroundDetection of the JAK2 mutation has recently been included under the essential diagnostic criteria for myeloproliferative neoplasm (MPN). High-resolution melt (HRM) curve analysis, a nongel-based, automated system, is introduced as a means of mutation scanning without the requirement of any post-PCR handling.MethodsWe studied the sensitivity and reproducibility of LightScanner™ platform in the detection of JAK2 V617F mutation and the availability for diagnostic use in MPN.ResultsThe reproducible sensitivity of HRM analysis with LightScanner™ platform was 5% for the detection of JAK2 V617F mutation. In the test of blind screening of 105 samples (48 Ph− MPN and 57 Ph+ chronic myeloid leukemia), the identical judgement was interpreted by two blinded investigators. HRM analysis of all cases was fully concordant with the results of PCR-RFLP and direct sequencing.ConclusionsThe HRM method developed here is an extremely sensitive, accurate and reliable technique and allows high-throughput, fast pre-screening to select for sequencing only those specimens that most likely contain mutant JAK2 V617F allele(s).
Journal: Clinica Chimica Acta - Volume 411, Issues 23–24, 14 December 2010, Pages 2097–2100