کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1966211 1538728 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Inter-α-trypsin inhibitor heavy chain 4 is a novel marker of acute ischemic stroke
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Inter-α-trypsin inhibitor heavy chain 4 is a novel marker of acute ischemic stroke
چکیده انگلیسی

BackgroundInflammation and inflammatory markers play an important role in acute ischemic stroke (AIS). In an earlier study, we discovered a 120-kDa protein that was highly expressed in healthy participants, but either was not expressed or was barely expressed in AIS patients. The purpose of the present work was to isolate, characterize, and evaluate this 120-kDa protein in blood samples of AIS patients. In addition, we investigated how the identified protein compared with protein S-100ßß, neuron-specific enolase (NSE), and cytokine interleukins IL-2 and IL-10.MethodsThe 120-kDa protein band was analyzed via LC-MS/MS analysis. Then, the 120-kDa protein was evaluated using an enzyme-linked immunosorbant assay in serum samples from AIS patients, which were collected 0, 24, 48, 72 and 144 h after admission.ResultsThe amino acid sequence of an internal fragment identified the 120-kDa protein as inter-α-trypsin inhibitor heavy chain 4 (ITIH4). The ITIH4 protein was completely absent in AIS patients as compared to those in the control group, and serum levels returned to normal in AIS patients as their condition improved. Expression of S-100 ßß, NSE, IL-2, and IL-10 were highly correlated with ITIH4 expression.ConclusionsITIH4 is an anti-inflammatory protein, and suggests that further investigation into its potential use in the diagnosis and prognosis of AIS is warranted.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinica Chimica Acta - Volume 402, Issues 1–2, April 2009, Pages 160–163
نویسندگان
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