کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1966317 1538729 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Increased perimeter red cell concentration in filter paper bloodspot samples is consistent with constant-load size exclusion chromatography occurring during application
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Increased perimeter red cell concentration in filter paper bloodspot samples is consistent with constant-load size exclusion chromatography occurring during application
چکیده انگلیسی

BackgroundFilter paper bloodspot samples exhibit increased red cell concentration near the bloodspot perimeter compared to the interior. We examined whether simulation of size exclusion chromatography separating cell and liquid components during bloodspot formation was consistent with the observed red cell distribution.MethodsWhole blood was defined as a mixture of 60% liquid and 40% solids (hematocrit). Bloodspot formation was simulated by step-wise center additions of 1 μl blood up to a total volume of 50 μl. Partitioning of the liquid component of the liquid volume into space not available to the solid component was calculated using a partitioning coefficient K = Vi / Vm, where Vi is the immobile (stationary) liquid volume and Vm is the mobile liquid volume. After each volume addition step, relative red cell concentration was calculated as the solids volume fraction of total volume as a function of radial distance from the center.ResultsSimulation for K = 0.3 resulted in final red cell distribution that was quantitatively consistent with bloodspot data.ConclusionsIncreased perimeter red cell concentration in bloodspots is consistent with partitioning of the liquid fraction into mobile and immobile components during bloodspot formation according to the principles of constant-load size exclusion chromatography.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinica Chimica Acta - Volume 401, Issues 1–2, March 2009, Pages 42–45
نویسندگان
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