کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1966468 1538722 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Circulating cell-free DNA levels in plasma increase with severity in experimental acute pulmonary thromboembolism
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Circulating cell-free DNA levels in plasma increase with severity in experimental acute pulmonary thromboembolism
چکیده انگلیسی

BackgroundThe diagnosis of acute pulmonary thromboembolism (APT) and its severity is challenging. No previous study has examined whether there is a linear relation between plasma DNA concentrations and the severity of APT. We examined this hypothesis in anesthetized dogs. We also examined the changes in plasma DNA concentrations in microspheres lung embolization and whether the therapy of APT with nitrite could modify APT-induced changes in plasma DNA concentrations. In vitro DNA release from blood clots was also studied.MethodsAPT was induced with autologous blood clots (saline, 1, 3, or 5 ml/kg) injected into the right atrium. A group of dogs received 300 µm microspheres into the inferior vena cava to produce similar pulmonary hypertension. Another group of dogs received 6.75 µmol/kg nitrite after APT with blood clots of 5 ml/kg. Hemodynamic evaluations were carried out for 120 min. DNA was extracted from plasma samples using QIAamp DNA Blood Mini Kit and quantified using Quant-iT™ PicoGreen® dsDNA detection kit at baseline and 120 min after APT.ResultsAPT produced dose-dependent increases in plasma DNA concentrations, which correlated positively with pulmonary vascular resistance (P = 0.002, r = 0.897) and with mean pulmonary arterial pressure (P = 0.006, r = 0.856). Conversely, lung embolization with microspheres produced no significant changes in plasma DNA concentrations. While nitrite attenuated APT-induced pulmonary hypertension, it produced no changes in plasma DNA concentrations. Blood clots released dose-dependent amounts of DNA in vitro.ConclusionsCell-free DNA concentrations increase in proportion to the severity of APT, probably as a result of increasing amounts of thrombi obstructing the pulmonary vessels.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinica Chimica Acta - Volume 409, Issues 1–2, 3 November 2009, Pages 112–116
نویسندگان
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