کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1966504 1538723 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Haptoglobin polymorphism in Mongolian population: Comparison of the two genotyping methods
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Haptoglobin polymorphism in Mongolian population: Comparison of the two genotyping methods
چکیده انگلیسی

BackgroundHaptoglobin (Hp) polymorphisms have been suggested to be associated with many pathological conditions including cardiovascular diseases, infectious diseases, and type 2 diabetes.MethodsWe examined the association between HP genotypes and type 2 diabetes or anthropometric and clinical features in 946 Mongolians. HP genotypes were determined by two methods, TaqMan-based real-time PCR and conventional PCR. Hp phenotyping was also performed by polyacrylamide gel electrophoresis and peroxidase staining in a few selected samples.ResultsThe 2 HP genotyping methods showed consistent results in 943 of 946 samples. Three samples that showed different results in the 2 PCR methods were determined to be heterozygous for the Hp Johnson allele by phenotyping. We did not find any association between HP genotypes and type 2 diabetes or the anthropometric and clinical features examined in the population studied.ConclusionsTaqMan-based HP genotyping is reliable and will be useful for high-throughput association studies. Further studies of large numbers of subjects and functional differences in Hp types are needed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinica Chimica Acta - Volume 408, Issues 1–2, 1 October 2009, Pages 110–113
نویسندگان
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