کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1966574 1538725 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Qualitative point-of-care and over-the-counter urine hCG devices differentially detect the hCG variants of early pregnancy
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Qualitative point-of-care and over-the-counter urine hCG devices differentially detect the hCG variants of early pregnancy
چکیده انگلیسی

BackgroundQualitative point-of-care (POC) tests for human chorionic gonadotropin (hCG) vary in their ability to detect purified hCG variants and there is data to suggest that over-the-counter (OTC) devices might also display similar variability. This could potentially influence the detection of urine hCG in early pregnancy.MethodsSix OTC devices were tested for their ability to detect 5 hCG variants. Ten early pregnancy urine specimens were selected for their diverse expression of hCG variants. The samples were tested with 6 brands of POC and 6 OTC devices.ResultsOTC devices consistently recognized intact hCG, hCGn, and hCGβ. hCGβn was consistently recognized by 4 out of 6 brands. One brand inconsistently recognized hCGβcf. OTC and POC devices varied greatly in their ability to detect hCG in early pregnancy urine, despite the fact that urine samples were adjusted to the same intact hCG concentration. Interestingly, we found that the OTC devices had better analytical sensitivity than the POC devices. Clinitest® and First Response® demonstrated the lowest hCG detection limits for POC and OTC devices, respectively.ConclusionsBoth OTC and POC devices are capable of detecting hCG concentrations in early pregnancy urine, and OTC devices demonstrated better analytical sensitivity relative to POC devices.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinica Chimica Acta - Volume 406, Issues 1–2, 11 August 2009, Pages 81–85
نویسندگان
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