کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1967909 | 1538753 | 2007 | 7 صفحه PDF | دانلود رایگان |
BackgroundCholesteryl ester transfer protein (CETP) is suggested to be involved in the cholesterol level in remnant like lipoprotein particles (RLP), but there is no direct evidence that CETP increases cholesterol-rich RLP in plasma.MethodsHuman plasma was incubated with or without HDL containing [3H]-labeled cholesteryl ester ([3H]CE), recombinant CETP or CETP inhibitors at 37 °C in vitro.ResultsThe RLP–cholesterol (RLP–C) level increased time-dependently and the amount of RLP–C increase (ΔRLP–C) by the incubation was positively correlated with triglyceride (TG) level in plasma (r = 0.597, P = 0.0070). [3H]CE in HDL was transferred to RLP fraction under 37 °C incubation, and the amount of [3H]CE transferred to RLP correlated significantly with ΔRLP–C in plasma (r = 0.611, P = 0.0156). Human recombinant CETP enhanced the RLP–C increase, while CETP inhibitor JTT-705 and anti-human CETP monoclonal antibody inhibited both the RLP–C increase and [3H]CE transfer to RLP. On the other hand, an inhibition of lecithin: cholesterol acyltransferase (LCAT) did not affect the RLP–C increase. In triglyceride-rich lipoproteins (TRL) fraction, JTT-705 inhibited [3H]CE transfer to RLP more strongly than that to non-RLP.ConclusionsCETP promotes the formation of cholesterol-rich RLP through the transfer of CE from HDL to TRL and CETP inhibitors are useful to reduce RLP–C.
Journal: Clinica Chimica Acta - Volume 375, Issues 1–2, January 2007, Pages 92–98