کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1968292 1538764 2006 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Real-time assays with molecular beacons and other fluorescent nucleic acid hybridization probes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Real-time assays with molecular beacons and other fluorescent nucleic acid hybridization probes
چکیده انگلیسی

BackgroundA number of formats for nucleic acid hybridization have been developed to identify DNA and RNA sequences that are involved in cellular processes and that aid in the diagnosis of genetic and infectious diseases.MethodsThe introduction of hybridization probes with interactive fluorophore pairs has enabled the development of homogeneous hybridization assays for the direct identification of nucleic acids. A change in the fluorescence of these probes indicates the presence of a target nucleic acid, and there is no need to separate unbound probes from hybridized probes.ConclusionsThe advantages of homogeneous hybridization assays are their speed and simplicity. In addition, homogeneous assays can be combined with nucleic acid amplification, enabling the detection of rare target nucleic acids. These assays can be followed in real time, providing quantitative determination of target nucleic acids over a broad range of concentrations.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinica Chimica Acta - Volume 363, Issues 1–2, January 2006, Pages 48–60
نویسندگان
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