Plasma homocysteine thiolactone adducts associated with risk of coronary heart disease

BackgroundHomocysteine thiolactone adducts have been proposed as the culprit of homocysteine related cardiovascular diseases. We studied the association of these adducts in plasma, and the gene polymorphism of paraoxonase-2 with coronary heart disease.Methods254 patients and 308 controls were recruited for the study. Homocysteine thiolactone adducts were determined with ELISA. The codon 311 polymorphism of paraoxonase-2 gene was genotyped by using polymerase chain reaction and restrictive digestion.ResultsThe plasma level of homocysteine thiolactone adducts were significantly higher in patients than in controls (40.65 ± 10.87 u/ml vs. 30.58 ± 10.20 u/ml, P < 0.01), with odds ratio of 7.34 (95% confidence interval 4.020∼13.406, P < 0.01), and increased according to the number of atherosclerotic coronary arteries: 35.59 ± 10.34 units/ml (n = 76); 41.88 ± 8.83 (n = 70) and 43.13 ± 11.47 (n = 108) in subjects with 1, 2 and 3 affected arteries, respectively (r = 0.174, P < 0.01). The frequency of CC genotype was significantly higher in patients with coronary heart disease (7.48%) than in controls (1.62%, P < 0.01), with adjusted odds ratio of 4.367 (95% confidence interval: 1.178 to 16.191, P < 0.01), so was the C allele (23.2% vs. 14.9%, P < 0.05).ConclusionsHigh plasma homocysteine thiolactone adducts and the CC 311 genotype of paraoxonase-2 gene may be the emerging risk factor for coronary heart disease.