کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1968342 | 1538763 | 2006 | 4 صفحه PDF | دانلود رایگان |

BackgroundThe measurement of morphine in biological samples has become a routine in many clinical and forensic toxicology laboratories. A high-performance liquid chromatography (HPLC) method was developed for the determination of morphine in plasma.MethodsSamples were extracted using Zeolite Y column followed by reversed phase HPLC with fluorescence detection. This method was based on an ex-calibration procedure and was linear between 20 and 200 ng/ml of morphine. Blood from 10 male opiate addicts were obtained from Rosbeh Hospital. All of the male smoked opiate (heroin, opium) and cigarettes.ResultsThe mean total level 5 h after the last abuse was 152.4 ng/ml and 37.6 ng/ml at 10–15 h. The method was reliable for morphine determination in blood even after 5 half-lives after the last abuse.ConclusionsThis method is simple and rapid and may be useful for routine monitoring of plasma morphine concentration.
Journal: Clinica Chimica Acta - Volume 364, Issues 1–2, February 2006, Pages 235–238