Application of 1H NMR metabonomics in predicting renal function recoverability after the relief of obstructive uropathy in adult patients

ObjectiveTo evaluate a method for predicting renal function recoverability after the relief of obstructive uropathy using 1H NMR-based metabonomics.Design and methodsThis study included 16 bilateral ureteral obstruction (BUO) patients and 9 unilateral ureteral obstruction (UUO) patients. The obstructions in all of the patients were successfully relieved after treatment. Urine samples at different time points before and after treatment were obtained, and their 1H NMR spectra were recorded and analyzed using multivariate statistical techniques combined with quantitative statistical analyses.ResultsA distinctive pattern in the metabolite profile of urine samples from preoperation and early and late stages after treatment was observed, which was in good agreement with the clinical chemistry analyses. Some important systemic perturbations in endogenous metabolites were correlated with different recoverability stages of renal function after obstruction relief. In addition, the OPLS-DA model of BUO patients could help predict the recovery of renal function of UUO patients.ConclusionThe results suggest that 1H NMR-based metabonomics can be used to evaluate the recoverability of renal function after obstruction relief. Our results suggest that 1H NMR-based metabonomics may help stratify patients according to disease stages, provide prognostic information, and be useful for postoperative surveillance.

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► 1H NMR-based metabonomics was used to predict the recoverability of renal function.
► The metabolic profile of urine from pre- and post-operation shows quite different.
► Altered metabolites are correlated with different recoverability stages after surgery.
► The metabonomics results are in good agreement with the clinical chemistry assay.
► The renal function of UUO patients could be predicted by the metabonomics approach.