Development of a novel enzyme-linked immunosorbent assay (ELISA) for measurement of serum CTRP1: A pilot study: Measurement of serum CTRP1 in healthy donors and patients with metabolic syndrome

BackgroundComplement C1q tumor necrosis factor-related protein 1 (CTRP1), a recently identified adipokine, was found to stimulate aldosterone production. Obesity and metabolic syndrome are frequently associated with elevated levels of aldosterone. Therefore, it would be interesting to investigate whether the secretion of CTRP1 in human serum is associated with obesity as well as with hypertension.AimThis study evaluated serum CTRP1 concentrations in healthy individuals and patients with metabolic syndrome.MethodsSerum samples from 61 healthy individuals and 46 patients with metabolic syndrome were measured for CTRP1 by enzyme-linked immunosorbent assay (ELISA).ResultsCorrelation analyses showed that serum CTRP1 in healthy individuals did not correlate with BMI, leptin, TG, HDL-CH, and LDL-CH; however, in patients with metabolic syndrome, CTRP1 correlated with glucose, HbA1c and BMI. CTRP1 level was significantly higher in subjects with metabolic syndrome compared to healthy subjects.DiscussionOur results support the hypothesis that adipokine CTRP1 is associated with metabolic syndrome and obesity compared to healthy individuals.

► We measured serum CTRP1 concentrations in patients with metabolic syndrome and in controls.
► We employed a novel ELISA with antibodies specific for CTRP1.
► The CTRP1 concentration in serum of patients with metabolic syndrome is significant higher than in serum of control group.
► We suggest that the adipokine CTRP1 is a marker of metabolic syndrome.