کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1969457 | 1538890 | 2011 | 6 صفحه PDF | دانلود رایگان |

ObjectiveThe development of a cytokine detection assay suitable for detection of multiple biomarkers for improved diagnosis of mycobacterial diseases.Design and methodsA lateral flow (LF) assay to detect IL-10 was developed utilizing the up-converting phosphor (UCP) reporter-technology. The assay was evaluated using blood samples of leprosy patients. Multiplex applications were explored targeting: 1) IL-10 and IFN-γ in assay buffer; 2) IL-10 and anti-phenolic glycolipid (PGL-I) antibodies in serum from leprosy patients.ResultsDetection of IL-10 below the targeted level of 100 pg/mL in serum was shown. Comparison with ELISA showed a quantitative correlation with R2 value of 0.92. Multiplexing of cytokines and simultaneous detection of cytokine and antibody was demonstrated.ConclusionsThe UCP-LF IL-10 assay is a user-friendly, rapid alternative for IL-10 ELISAs, suitable for multiplex detection of different cytokines and can be merged with antibody-detection assays to simultaneously detect cellular- and humoral immunity.
Journal: Clinical Biochemistry - Volume 44, Issues 14–15, October 2011, Pages 1241–1246