کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1969498 | 1538893 | 2011 | 6 صفحه PDF | دانلود رایگان |
ObjectivesThe aim of this study was to evaluate the suitability of chemiluminescent enzyme immunoassay (CLIA) for the monitoring of whole-blood tacrolimus concentrations in rheumatoid arthritis (RA) patients.Design and methodsSixty-three RA patients and 47 renal transplant (RT) patients treated with tacrolimus were enrolled. Tacrolimus concentrations in spiked blood and patient blood were measured by CLIA and HPLC-MS/MS. The cross-reactivity in CLIA was evaluated using 13-O-demethylated or 31-O-demethylated tacrolimus.ResultsTacrolimus concentrations measured by CLIA correlated with those measured by HPLC-MS/MS. Bland–Altman analysis revealed the 95% confidence intervals between CLIA and HPLC-MS/MS in RA and RT patients were − 20.7 to 109.9% and − 5.0 to 74.1%, respectively. While 31-O-demethylated tacrolimus cross-reaction amounted to an equivalent of 120% tacrolimus in CLIA, 13-O-demethylated tacrolimus did not cross-react.ConclusionCLIA values should be carefully interpreted in RA patients, especially those receiving a low dose of tacrolimus.
Journal: Clinical Biochemistry - Volume 44, Issues 5–6, April 2011, Pages 397–402