کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1969736 | 1059779 | 2011 | 6 صفحه PDF | دانلود رایگان |
ObjectivesCarbonylation of the protein amino, guanidino and thiol groups is one of the important causes of vascular complications in diabetes. We developed a simple spectrophotometric method for monitoring of the changes in the protein amino group contents during carbonylation.Design and methodsThe method is based on the reaction of amino group with p-benzoquinone in the slightly alkaline media. It was applied during carbonylation in vitro with methylglyoxal and in vivo in 13 patients with type 2 diabetes and 20 healthy persons.ResultsThe method is simple, fast, precise (RSD in the range of 1.2–1.8%) and accurate (recovery about 100%). The content of amino groups in human serum albumin isolated from diabetics was significantly lower (p < 0.01) in comparison with a control group.ConclusionThe method developed is suitable for quantification of protein amino groups during in vitro carbonylation as well as for clinical practice.
Journal: Clinical Biochemistry - Volume 44, Issue 12, August 2011, Pages 994–999