کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1970021 1538900 2010 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
SELDI-TOF biomarker signatures for cystic fibrosis, asthma and chronic obstructive pulmonary disease
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
SELDI-TOF biomarker signatures for cystic fibrosis, asthma and chronic obstructive pulmonary disease
چکیده انگلیسی

Objectives:The aim of this work was to establish protein profiles in serum and nasal epithelial cells of cystic fibrosis individuals in comparison with controls, asthma and chronic obstructive pulmonary disease patients for specific biomarker signatures identification.Design and methods:Protein extracts were analyzed by Surface Enhanced Laser Desorption/Ionization Time-Of-Flight Mass-Spectrometry (SELDI-TOF-MS).Results:The mass spectra revealed a set of peaks with differential expression in serum and nasal cells among the different groups studied, resulting into peak signatures representative/specific of each pathology. Logistic regressions were applied to those peaks; sensitivity, specificity, Youden's indexes and area under the curve (AUC) of the respective receiver operating characteristic (ROC) curves were compared.Discussion:Multivariate analysis demonstrated that combination of peaks has a better predictive value than the individual ones. These protein signatures may serve as diagnostic/prognostic markers for the studied diseases with common clinical features, or as follow-up assessment markers of therapeutic interventions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinical Biochemistry - Volume 43, Issues 1–2, January 2010, Pages 168–177
نویسندگان
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