کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1970702 | 1059815 | 2006 | 7 صفحه PDF | دانلود رایگان |

Objectives:Novel comparison of CDT isoforms as determined by CE with an FDA-approved immunoassay kit.Design and methods:Subjects (n = 51) were categorized by drinking status based on AUDIT questionnaire responses. CDT isoform analyses by CE were compared to a commercially available, FDA-approved immunoassay. The analytical specificity of the immunoassay kit was assessed by analysis with IEF.Results:Because of the poor correlation between % CDT as measured by CE and the TIA immunoassay and between subject-reported drinking levels and results from the TIA assay, extraction column eluants from the immunoassays were analyzed by IEF for analytical specificity. % CDT by TIA included some trisialo-Tf, a non-CDT fraction, in the % CDT determination.Conclusions:Total % CDT by CE, which separates all isoforms is more analytically specific than immunoassays because it does not include trisialo-Tf in the CDT calculation.
Journal: Clinical Biochemistry - Volume 39, Issue 8, August 2006, Pages 826–832