کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1970703 1059815 2006 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Detection of known thalassemia point mutations by snapback single-strand conformation polymorphism: The feasibility analysis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Detection of known thalassemia point mutations by snapback single-strand conformation polymorphism: The feasibility analysis
چکیده انگلیسی
Snapback SSCP can be applied to the detection of known thalassemia point mutations. The advantages of snapback SSCP are (1) it is simple as compared to PCR-ASO; (2) snapback SSCP is specific and stable once the conditions of snapback SSCP are optimized; (3) samples can be checked at any time without the limit of half-life of radioactive isotope; and (4) since PCR products used in snapback SSCP can be checked by using 2% agarose gel before snapback SSCP, misdiagnoses caused by false positive or false negative PCR can be reduced significantly in snapback SSCP.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinical Biochemistry - Volume 39, Issue 8, August 2006, Pages 833-842
نویسندگان
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