کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1971664 1538911 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Competitive and product inhibition-based α-amylase activity analysis method
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Competitive and product inhibition-based α-amylase activity analysis method
چکیده انگلیسی

ObjectivesTo propose a methodology for analyzing salivary α-amylase activity (sAMY) for a hand-held device that can be used easily and quickly for evaluating human psychological effects.MethodsAn improved method for the analysis of sAMY is proposed using competitive and product inhibition in a dry-chemistry system with a reagent paper containing 2-chloro-4-nitrophenyl-4-O-β-d-galactopyranosylmaltoside.ResultsNot only the competitive (maltopentaose) inhibitor, but also the product (maltotriose) inhibitor significantly reduce the reaction speed of sAMY. There is an independent effect between the two inhibitors. The dynamic linear range of the analysis can be enhanced by 2.5 times larger by adding the competitive and product inhibitors simultaneously and by preparing their concentrations appropriately.ConclusionsIt is considered that the application of competitive and product inhibition can be effective from the viewpoint of enhancing analysis range and effectively reducing costs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinical Biochemistry - Volume 41, Issues 4–5, March 2008, Pages 325–330
نویسندگان
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