Antiviral activity of the inducible humoral immunity and its suppression by eleven BEN family members encoded in Cotesia plutellae bracovirus

Upon parasitization by some endoparasitoids, polydnaviruses (PDVs) play a crucial role in inducing host immunosuppression. This study reports a novel immunosuppressive activity against humoral immune responses by BEN family genes encoded in Cotesia plutellae bracovirus (CpBV). A total of 11 BEN family members are encoded in 10 different CpBV DNA segments. When the CpBV segments were individually injected, specific BEN genes were expressed and suppressed the expression of antimicrobial peptide (AMP) and prophenoloxidase genes following bacterial challenge. The suppressive activities of the BEN genes were reversed by injection of the double-stranded RNA (dsRNA) specific to each BEN gene. The suppression of the AMP gene expressions by the BEN genes was also confirmed using an inhibition zone assay against Gram-positive and Gram-negative bacterial growth. The significance of the suppressive activity of BEN genes against humoral immune responses was analyzed in terms of suppression of antiviral activity by the host humoral immunity. When CpBV was incubated with the plasma obtained from the larvae challenged with bacteria, the immunized plasma severely impaired the expression activity of the viral genes. However, an expression of BEN gene significantly rescued the viral gene expression by suppressing humoral immune response. These results suggest that BEN family genes of CpBV play a crucial role in defending the antiviral response of the parasitized Plutella xylostella by inhibiting humoral immune responses.