کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1972355 1060273 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
cDNA cloning, characterization and expression of lipopolysaccharide and β-1,3-glucan binding protein (LGBP) gene from the Indian white shrimp Fenneropenaeus indicus
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
cDNA cloning, characterization and expression of lipopolysaccharide and β-1,3-glucan binding protein (LGBP) gene from the Indian white shrimp Fenneropenaeus indicus
چکیده انگلیسی

A 1372 bp full length cDNA of an LGBP gene was identified from the Indian white shrimp, Fenneropenaeus indicus. The open reading frame (ORF) of the F. indicus LGBP (Fein-LGBP) was 1164 bp long, encoding a polypeptide of 388 amino acids. The Fein-LGBP deduced amino acid has conserved potential recognition motif for β-1, 3 linkages of polysaccharides and putative RGD (Arg–Gly–Asp) cell adhesion sites. Phylogenetic analysis revealed that the Fein-LGBP was grouped together with LGBPs of other crustaceans such as F. chinensis (91%, AAZ41363), Penaeus monodon (88%, AAM21213) and Litopenaeus vannamei (88%, ABU92557). Quantitative RT-PCR results showed that Fein-LGBP gene expression was up-regulated in hemocytes following a 6 h challenge in response to peptidoglycan (PG) and Vibrio parahaemolyticus. Messenger RNA transcripts of Fein-LGBP were measured in hemocytes of F. indicus from different molting stages. The highest Fein-LGBP mRNA expression was observed in pre-molting stages (D0/1) when compared to post-molt stages (A and B) and inter-molt stages (C). Fein-LGBP is involved in the regulation and activation of the prophenoloxidase cascade.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 163, Issue 1, September 2012, Pages 74–81
نویسندگان
, ,