کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1975275 | 1060612 | 2013 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular cloning of pepsinogens A and C from adult newt (Cynops pyrrhogaster) stomach
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Molecular cloning of pepsinogens A and C from adult newt (Cynops pyrrhogaster) stomach Molecular cloning of pepsinogens A and C from adult newt (Cynops pyrrhogaster) stomach](/preview/png/1975275.png)
چکیده انگلیسی
The full-length cDNAs of three pepsinogens (Pgs) were cloned from the stomach of newt, Cynops pyrrhogaster, and nucleotide sequences of the full-length cDNAs were determined. Molecular phylogenetic analysis showed that two Pgs, named PgC1 and PgC2, belong to the pepsinogen C group, and one Pg, named PgA, belongs to the pepsinogen A group. The sequences contain an open reading frame (ORF) encoding 385 amino acid residues for PgC1, 383 amino acid residues for PgC2 and 377 amino acid residues for PgA. In addition, all of the three amino acid sequences conserve some unique characteristics such as six cysteine residues and putative active site two aspartic acid residues. All of the pepsinogen mRNAs were detected in the stomach by RT-PCR but not in other organs. Although a slight difference at the time of the start of expression was seen among the three pepsinogen genes, all of them were expressed in the larval stage after hatching. This is the first report on cloning of pepsinogens from urodele stomach.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 165, Issue 4, August 2013, Pages 226-235
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 165, Issue 4, August 2013, Pages 226-235
نویسندگان
Tomofumi Inokuchi, Masayuki Ikuzawa, Shin Yamazaki, Yukari Watanabe, Koushiro Shiota, Takuma Katoh, Ken-ichiro Kobayashi,