کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1975302 | 1060614 | 2013 | 9 صفحه PDF | دانلود رایگان |
Fatty acid delta-6 desaturase (fads2)-like gene from Nile tilapia (Oreochromis niloticus) was characterized and designated as oni-fads2. The Oni-FADS2 showed the typical structure of microsomal FADS2. The presence of oni-fads2 transcripts in unfertilized eggs demonstrated the maternal role of Nile tilapia in providing the oni-fads2 transcript in their eggs. In addition, the expression of oni-fads2 was detectable in embryos throughout the hatching stage. Real-time reverse transcription-PCR revealed that oni-fads2 was expressed at a high level in all the brain regions, liver, and testis. Recombinant yeast (RY) was generated by transformation of Saccharomyces cerevisiae with the plasmid containing oni-fads2 driven by the Gal1 promoter (pYoni-fads2). The conspicuous expression of RY was detectable by RT-PCR after induction with galactose for 24 h. When RY was induced with galactose, it exhibited 39% and 7% of delta-6 desaturase (∆6) activity toward C18:2n6 and C18:3n3, respectively. Additionally, it displayed 4% of delta-5 desaturase (∆5) activity toward C20:3n6, indicating that Oni-FADS2 had ∆5 and ∆6 bifunction.
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 166, Issue 2, October 2013, Pages 148–156