کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1975936 1060665 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Functional characterization of a masquerade-like serine proteinase homologue from the black tiger shrimp Penaeus monodon
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Functional characterization of a masquerade-like serine proteinase homologue from the black tiger shrimp Penaeus monodon
چکیده انگلیسی
A cDNA encoding a masquerade-like serine proteinase homologue (PmMasSPH) from the black tiger shrimp, Penaeus monodon, has been cloned and characterized. The transcript of PmMasSPH is induced in response to Vibrio harveyi infection. To further characterize the function(s) of the protein, (i) the N-terminal region comprising the glycine-rich repeats and the clip domain, and (ii) the C-terminal SP-like domain of the PmMasSPH were separately cloned into the pET-28b(+) expression vector and transformed into Escherichia coli Rosetta (DE3). The two recombinant proteins were then assayed for various biological functions; proteinase activity, hemocyte adhesion, bacterial binding, bacterial clearance and antimicrobial activity. The C-terminal SP-like domain lacks proteolytic activity but mediates hemocyte adhesion and displays binding activity to the shrimp pathogenic bacterium, V. harveyi and specific binding to the bacterial cell wall component, lipopolysaccharide (LPS). The N-terminal region exhibited in vitro antimicrobial activity against Gram-positive bacteria. In addition, the in vivo study revealed the opsonic activity of the PmMasSPH protein as shown by a higher bacterial clearance rate of V. harveyi coated with the recombinant proteins as compared with V. harveyi only. The results suggest that the PmMasSPH protein is a multifunctional immune molecule in shrimp defense.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 153, Issue 3, July 2009, Pages 236-243
نویسندگان
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