Translation inhibitory factors encoded in Cotesia plutellae bracovirus require the 5′-UTR of a host mRNA target

Physiological processes of the diamondback moth, Plutella xylostella, larvae parasitized by Cotesia plutellae are altered by several parasitic factors including a polydnavirus, C. plutellae bracovirus (CpBV). Two homologous genes, CpBV15α and CpBV15β, have been proposed as host translation inhibitory factors (HTIFs). This study analyzed their effects on host gene expression at a post-transcriptional level. A proteomic approach using two dimensional electrophoresis revealed that the parasitization resulted in 24.0% (60/250 spots) reduction of gene expression compared to nonparasitized control. It also indicated that the transient expression of CpBV15α or CpBV15β in nonparasitized larvae resulted in 26.0% (65/240 spots) or 28.0% (70/240 spots) reduction, respectively. Seven spots that were not detected in the transiently expressed samples were further analyzed by a tandem mass spectrometry. These proteins were predicted to be associated with host cell signaling and metabolism. To investigate translation inhibitory effects of CpBV15α and CpBV15β, capped mRNA of a storage protein 1 (SP1) of P. xylostella, a common inhibitory target of both HTIFs, was prepared by in vitro transcription and translated in vitro in the presence or absence of recombinant HTIFs prepared from Sf9 cells by recombinant baculoviruses. Translation of SP1 mRNA containing 5′-untranslated region (5′-UTR) was inhibited by both HTIFs. However, translation of SP1 mRNA without 5′-UTR was insensitive to the exposure of both HTIFs. Both HTIFs inhibited the host gene translation in a dose-dependent manner. In addition, these two factors showed cooperative inhibition. This study suggests that CpBV15α and CpBV15β inhibit host mRNAs directly by acting on translation machinery, in which 5′-UTR of target mRNAs would be required for the inhibitory action.