کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1976128 | 1060676 | 2008 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Vitellin-binding proteins in the nematode Oscheius tipulae (Nematoda, Rhabditida)
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Vitellin-binding proteins in the nematode Oscheius tipulae (Nematoda, Rhabditida) Vitellin-binding proteins in the nematode Oscheius tipulae (Nematoda, Rhabditida)](/preview/png/1976128.png)
چکیده انگلیسی
We describe the first application of a non-radioactive ligand-blotting technique to the characterization of proteins interacting with nematode vitellins. Chromatographically purified vitellins from the free-living nematode Oscheius tipulae were labeled with fluorescein in vitro. Ligand-blotting assays with horseradish peroxidase-conjugated anti-fluorescein antibodies showed that labeled vitellins reacted specifically with a polypeptide of approximately 100 kDa, which we named P100. This polypeptide is a specific worm's vitellin-binding protein that is present only in adult worms. Blots containing purified O. tipulae vitellin preparations showed no detectable signal in the 100 kDa region, ruling out any possibility of yolk polypeptides self-assembling under the conditions used in our assay. Experiments done in the presence of α-methyl mannoside ruled out the possibility of vitellins binding to P100 through mannose residues. Triton X-114 fractionation of whole worm extracts showed that P100 is either a membrane protein or has highly hydrophobic regions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 151, Issue 3, November 2008, Pages 330-335
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 151, Issue 3, November 2008, Pages 330-335
نویسندگان
João Carlos Jr., Daniela Peres Almenara, Cristiane Penha-Scarabotto, Joselene Pereira de Moura, Carlos Eduardo Winter,