کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1976213 | 1060681 | 2008 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Acetylcholinesterase in the sea urchin Lytechinus variegatus: Characterization and developmental expression in larvae
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Acetylcholinesterase (AChE) in the echinoid Lytechinus variegatus has been characterized. Kinetic parameters Vmax, Km, Kss, and b were 2594 ± 1048 nmol ATCh hydrolyzed/min/mg tissue wet weight, 185 ± 11 μM, 308 ± 100 mM, and 0.2, respectively for the substrate ATCh and 17.8 ± 6.87 nmol BTCh hydrolyzed/min/mg tissue wet weight, 654 ± 424 μM, 36 ± 31 mM, and 0.6, respectively for BTCh. Pharmacologic analyses were performed with four inhibitors of cholinesterases, physostigmine, BW284c51, ethopropazine, and iso-OMPA, and yielded IC50 values of 106 ± 4 nM, 718 ± 118 nM, 2.57 ± 0.6 mM, and > 0.0300 M, respectively. Both kinetic and pharmacologic results confirmed the existence of AChE in larval L. variegatus. Dimeric and tetrameric globular forms (determined by velocity sedimentation on sucrose gradients) were present in L. variegatus larvae. Activity of AChE increased significantly as larvae progressed in development from embryos to eight-arm larvae. Acetylcholinesterase activity of F1 larvae derived from sea urchins collected from wild populations and of F1 larvae derived from sea urchins cultured in the laboratory and fed two different diets suggest that the nutritional and/or environmental history of the adult sea urchin affect the developmental progression of AChE activity in the F1 offspring.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 149, Issue 3, March 2008, Pages 401-409
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 149, Issue 3, March 2008, Pages 401-409
نویسندگان
Natalie A. Jennings, Leo Pezzementi, Addison L. Lawrence, Stephen A. Watts,