کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1977177 | 1539280 | 2016 | 8 صفحه PDF | دانلود رایگان |
Environmental stress may alter the bioenergetic balance of organisms by resulting in greater energy investment into detoxification processes, which diverts energy from other biological functions. Here, we examine responses to triclosan (TCS) exposure in a freshwater mussel across multiple biological levels: behavioral (e.g., burrowing and movement activity), organismal (e.g., metabolic rate and heart rate), and subcellular (e.g., gene expression and protein abundance/activity). At the subcellular level, we employed both energetic (i.e., AMP-activated protein kinase (AMPK)) and traditional (i.e., heat shock protein (HSP70), superoxide dismutase (SOD), glutathione-S-transferase (GST)) biomarkers. We found a significant reduction in burrowing and movement behaviors, a 1.8-fold increase in total-AMPK protein abundance, and a 2.8-fold increase in AMPK activity after 21 d. GST activity increased after 4 d, but not after 21 d. Our findings suggest that TCS exposure results in an energetic tradeoff between detoxification at the cellular level and whole-animal activity.
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 179, January 2016, Pages 150–157