کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1978639 1061613 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
De novo assembly and characterization of spotted seal Phoca largha transcriptome using Illumina paired-end sequencing
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
De novo assembly and characterization of spotted seal Phoca largha transcriptome using Illumina paired-end sequencing
چکیده انگلیسی

Spotted seal (Phoca largha) is categorized as a critically endangered species in China. The aim of this study was to investigate spotted seal transcriptome by the approach of Illumina paired-end sequencing technology. We obtained a total of 52,146,394 reads for the mixed tissues of liver and spleen from the spotted seal. The de novo assemblies yielded 354,014 contigs and 178,466 unigenes. In the transcriptome, 193 unigenes were assigned to defense mechanisms. Three unigenes encoded MHC class I and 17 unigenes encoded MHC class II. In addition, bioinformatics analysis revealed a total of 4425 simple sequence repeats (SSRs). Fifty SSRs were randomly selected to validate amplification and determine the degree of polymorphism in the genomic DNA pools. Thirty-five primer pairs successfully amplified the expected DNA fragments and detected significant polymorphism among 28 spotted seal individuals. These results would contribute to the understanding of the genetic makeup of spotted seal transcriptome and provide useful information for functional genomic research in this species.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - Volume 8, Issue 2, June 2013, Pages 103–110
نویسندگان
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