کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1978898 1061632 2007 15 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Proteomic analysis of early lactation milk of the tammar wallaby (Macropus eugenii)
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Proteomic analysis of early lactation milk of the tammar wallaby (Macropus eugenii)
چکیده انگلیسی

We report the successful use of 2D electrophoresis, MALDI MS/MS and chemical derivatisation protocols of guanidination and sulfonation to identify over 100 protein spots present in early marsupial milk (tammar wallaby) at 40 days lactation, where a limited translated genomic database is publicly available for cross species matching and protein identification. Of the proteins identified, 25 matched to 6 existing marsupial milk protein sequences in the NCBI database; another 6 were identified with high confidence to other mammals and have not previously been identified in marsupial milk. By using chemical derivatisation, the reliable identification of a further 81 proteins was achieved. The identified proteins could be grouped into three main functional categories — transport, nutrition and immune protection. All these proteins play a potential role in determining growth and immunological protection of the highly altricial marsupial young at 40 days after birth.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - Volume 2, Issue 2, June 2007, Pages 150–164
نویسندگان
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