کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1978999 1061638 2007 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Guanylyl cyclases in the tropical land crab, Gecarcinus lateralis: Cloning of soluble (NO-sensitive and -insensitive) and membrane receptor forms
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Guanylyl cyclases in the tropical land crab, Gecarcinus lateralis: Cloning of soluble (NO-sensitive and -insensitive) and membrane receptor forms
چکیده انگلیسی

cDNAs encoding three guanylyl cyclases (GCs), which catalyze the production cGMP from GTP, were cloned from the blackback land crab, Gecarcinus lateralis: the β subunit of a NO-sensitive soluble GC (Gl-GC-Iβ; 2380 bp; 603 amino acids; 68,435 Da), a membrane receptor GC (Gl-GC-II; 4609 bp; 1349 amino acids; 150,999 Da), and a NO-insensitive soluble GC (Gl-GC-III; 1416 bp; 285 amino acids; 32,487 Da). All three have a highly-conserved catalytic domain located in the C-terminal region and are therefore likely to be enzymatically active. Gl-GCIβ contains heme/NO-binding (HNOB) and HNOB-associated domains characteristic of the catalytic subunit of NO-sensitive GCs. Gl-GC-II encodes a single-pass membrane protein containing ligand-binding (LB), transmembrane (TM), kinase homology (KH), and dimerization domains. Gl-GC-III is similar to Gl-GC-II but lacks the LB, TM, and most of the KH domains. Isoforms of Gl-GC-Iβ and Gl-GC-II appear to be generated by alternative splicing. Two Gl-GC-Iβ isoforms differed in the absence (Δ32N) or presence (Δ0N) of a 32-amino acid sequence at the N-terminus. The truncated form may not bind a heme group, but still would be able to dimerize with the alpha subunit to form a NO-insensitive enzyme. Three Gl-GC-II isoforms varied in length within the N-terminal ligand-binding domain (+ 18, + 9, and + 0 amino acid insertions). As GC-II is the putative receptor for crustacean hyperglycemic hormone (CHH), these data suggest that the isoforms differ in binding to CHH and related neuropeptides.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - Volume 2, Issue 4, December 2007, Pages 332–344
نویسندگان
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