کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1979010 | 1061639 | 2006 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Genetic evidence for the existence of interleukin-23 and for variation in the interleukin-12 and interleukin-12 receptor genes in the horse Genetic evidence for the existence of interleukin-23 and for variation in the interleukin-12 and interleukin-12 receptor genes in the horse](/preview/png/1979010.png)
Immune loci, characterized by features reflecting their role in defense reactions and consequently related to evolutionary mechanisms, including polymorphisms or association with disease are suitable candidates for comparative analysis. Interleukin-12 and related cytokines are key molecules regulating natural and specific immune responses. In this study, we analyzed four horse IL12-related genes: IL23p19, IL12Rβ2, IL12p40, and IL12p35. Genomic nucleotide sequence of the horse IL23 p19 sub-unit encoding gene was determined. The horse IL23p19 gene consists of four exons; its total mRNA length is 1004 bp, with a coding region of 579 bp. The predicted amino acid sequence of the horse IL23p19 sub-unit showed 88.0% sequence identity with the human sequence. A partial genomic sequence highly homologous to human IL12Rβ2 suggesting existence of this gene in the horse was retrieved. Single nucleotide polymorphisms (SNPs) were identified in all four genes analyzed. PCR-RFLP genotyping was developed for selected SNPs. Inter-breed differences in allele and genotype frequencies were observed in IL12p35 SNP 242. The results showed that horse IL12-related genes are comparable to their counterparts in other mammalian species in terms of their structure and their genetic variation.
Journal: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - Volume 1, Issue 2, June 2006, Pages 179–186